myCONSTRUCT
Amino acid construct optimization - Rational multi-epitope construct design
myCONSTRUCT supports the rational design of multi-epitope, string-of-beads constructs. Because epitope assembly creates novel junctional peptides, the platform evaluates each junction for proteasomal processing, MHC presentation, and immunogenicity. It then optimizes epitope order and spacer selection to minimize unintended immune responses while preserving therapeutic focus. It returns several independently optimized candidate designs rather than a single construct, and can also begin from an existing construct.
What myCONSTRUCT delivers
String-of-beads design optimization with myCONSTRUCT has a turnaround time of one week:
- Junctional peptide generation: enumeration of all peptide fragments that could arise across the junction following intracellular processing
- Processing and presentation modeling: evaluation of each junction for proteasomal likelihood, MHC class I (and optionally class II) presentation probability, and allele-specific binding and presentation features
- Immunogenicity scoring: estimation of the probability a presented peptide will elicit a T-cell response
- Integrated junction risk aggregation: risk score aggregation across alleles, weighted by allele frequency, and summarized at construct level
- Design optimization: order refinement, spacer selection, and construct-level junction penalty minimization
- Multiple candidate designs: several independently optimized constructs rather than a single sequence
- Existing-construct optimization: start from a current design and refine epitope order and spacers
What is myCONSTRUCT?
myCONSTRUCT supports the rational design of multi-epitope, string-of-beads constructs. Because epitope assembly creates novel junctional peptides, the platform evaluates each junction for proteasomal processing, MHC presentation, and immunogenicity. It then optimizes epitope order and spacer selection to minimize unintended immune responses while preserving therapeutic focus.
What is the output of myCONSTRUCT?
myCONSTRUCT returns several independently optimized candidate designs rather than a single construct, reducing reliance on any one sequence. Each candidate can be assessed for the intended human context and relevant preclinical species, including mouse. The workflow can also begin from an existing construct and optimize its epitope order and spacers.
What is the turnaround time for myCONSTRUCT?
String-of-beads design optimization with myCONSTRUCT has a turnaround time of one week.